Journal of the Society for Gynecologic Investigation

 

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Journal of the Society for Gynecologic Investigation, Vol. 4, No. 4, 209-217 (1997)
DOI: 10.1177/107155769700400407

Gq-Protein Alpha Subunit Expression and Distribution in Pregnant Rat Myometrial Tissues

Cathleen M. Harris, MD

Department of Obstetrics and Gynecology, MARP 301, University of Chiacago, IL 60637

Trevania Saunders

David A. Baunoch, PhD

Department of Obstetrics and Gynecology, University of Chicago, Chicago, Illinois

Mark Phillippe, MD

Department of Obstetrics and Gynecology, M/C 2050, University of Chicago, 5841 S. Maryland, Chicago, IL 60637

Objective: Multiple G-protein isoforms play an integral role in signal transduction; the Gq subtype of G-protein alpha subunits is involved in the activation of the phosphatidylinositol signaling pathway. The studies described herein evaluate the expression of Gq, along with Gs and Gi, in pregnant and nonpregnant rat myometrial tissues.

Methods: Myometrium and other tissues were obtained from nonpregnant and timed-pergnant Sprangue-Dawley rats. Western blot studies were performed using polycclonal G-protein isoform-specific antibodies. Immunohistochemical studies were performed using the same antibodies with specimens of myometrium, intestine, and skeletal muscle.

Results: The Western blot studies confirmed differential expression of all of these G-protein {alpha} subunit subtypes in rat myometrial tissues. In pregnant rat myometrium, the expression of Gq and Gs was sustained through day 22, whereas, Gi expression decreased on day 20 and remained low through the remainder of gestation. The immunohistochemical studies revealed significant staining for Gq and Gs in the myometrial layers of the pregnant and nonpregnant rat uterus; in contrast, immunostaining for Gi was minimal in nonpregnant myometrium, and even lower in myometrium from pregnant uteri.

Conclusions: These studies have confirmed expression of the Gq, Gi, and Gs {alpha} subunits in rat myometrical tissue. Immunohistochemistry confirmed that Gq was expressed at high levels in the myometrial layer of the pregnant and nonpregnant uterus. These observations support the hypothesis that Gq expression is critically important for the transduction of hormone signals, such as those responsible for the generation of phasic myometrial contractions.

Key Words: Myometrium • G-proteins • Gq • phosphatidylinositol • adenylyl cyclase


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