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Journal of the Society for Gynecologic Investigation
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Expression of Interleukin-10 in Human Gestational Tissues

Michael S. Trautman, MD

Debbie Collmer, BS

Sam S. Edwin, BS

Departments of Pediatrics and Obstetrics and Gynecology, University of Utah; Geriatric Research Education Clinical Center, Salt Lake City Veterans Hospital, Salt Lake City, Utah

William White, BS

Departments of Pediatrics and Obstetrics and Gynecology, University of Utah; Geriatric Research Education Clinical Center, Salt Lake City Veterans Hospital, Salt Lake City, Utah; Division of Pulmonology, University of Utah, Salt Lake City, UT

Murray D. Mitchell, DPhil, DSc

Departments of Pediatrics and Obstetrics and Gynecology, University of Utah; Geriatric Research Education Clinical Center, Salt Lake City Veterans Hospital, Salt Lake City, Utah; Department of Pharmacology and Clinical Pharmacology, University of Auckland, New Zealand

Donald J. Dudley, MD

Departments of Pediatrics and Obstetrics and Gynecology, University of Utah; Geriatric Research Education Clinical Center, Salt Lake City Veterans Hospital, Salt Lake City, Utah

Objective: To determine production of interleukin-10 (IL-10) by interleukin-1ß (IL-1ß) in cultured decidual, chorion, and amnion cells and whether IL-10 is produced is gestational tissues under the setting of infection-associated preterm labor.

Methods: Decidual, chorion, and amnion cells were isolated from term placentas and grown in primary culture. The cells were incubated with various concentrations of IL-1ß and then culture supernatants were assayed for IL-10 by enzyme-linked immunosorbent assay. In subsequent studies, gestational membranes were isolated from a normal-term pregnancy and a preterm pregnancy complicated by chorioamnionitis. Tissues were evaluated for IL-10 expression by immunohistology and in situ hybridization. Human gestational tissues were collected from 38 women experiencing: 1) term cesarean delivery without labor; 2) normal-term vaginal delivery; 3) preterm cesarean delivery without labor; 4) preterm vaginal delivery without chorioamnionitis; and 5) preterm vaginal delivery with concomitant chorioamnionitis. Amnion, chorion, and decidua were isolated, total RNA from each tissue was extracted, and the presence of IL-10 mRNA was determined by reverse transcriptase-polymerase chain reaction (RT-PCR).

Results: Decidual cells in culture produced IL-10 in response to IL-1ß, but chorion and amnion cells produced no IL-10 protein. In vivo protein expression by immunohistology showed that most protein was detected within decidua while cells within amnion and chorion rarely had detectable IL-10 protein. In vivo RT-PCR samples demonstrated the strongest IL-10 mRNA signal from decidua samples, although IL-10 mRNA was also noted in chorion and amnion of placentas obtained after preterm labor.

Conclusion: Maternal decidual cells can potentially produce IL-10, but fetal membranes (amnion and chorion) appear to have limited capabilities to produce IL-10. The relative inability of fetal tissues to produce IL-10 may play an important role in the pathophysiology of infection-associated preterm labor.

Key Words: Interleukin-10 • cytokines • decidua • chorion • preterm labor

Journal of the Society for Gynecologic Investigation, Vol. 4, No. 5, 247-253 (1997)
DOI: 10.1177/107155769700400505
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