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Journal of the Society for Gynecologic Investigation
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Leuprolide Acetate-Treated Leiomyomas Retain Their Relative Overexpression of Collagen Type I and Collagen Type III Messenger Ribonucleic Acid

Elizabeth A. Stewart, MD

Department of Obstetrics, Gynecology and Reproductive Biology, Harvard Medical School and Brigham and Women's Hospital, Boston. Massachusetts; Laboratory for Human Reproduction and Reproductive Biology, Room 449, Boston Lying In, 221 Logwood Avenue, Boston, MA 02115. eastewart{at}bics.bwh.harvard.edu

Alan R. Rhoades, BS

Romana A. Nowak, PhD

Department of Obstetrics, Gynecology and Reproductive Biology, Harvard Medical School and Brigham and Women's Hospital, Boston. Massachusetts

Objective: To evaluate the levels of mRNA for the extracellular matrix proteins collagen type I, collagen type III, and fibronectin in leiomyomas and myometrium obtained from women treated with GnRH-agonist (GnRH-a) and to examine steroid hormone regulation of these proteins using an in vitro explant culture system.

Methods: Northern blot analysis of mRNA was obtained from hysterectomy specimens at the time of surgery or after 48 hours of in vitro steroid treatment. A portion of the tissue was processed for RNA, and the remaining tissue was used to establish explant cultures. Extracellular matrix/{alpha}-tubulin ratio was computed for each band, and within each experiment the lowest ratio was standardized to a value of one denstometry unit to allow for comparison among experiments.

Results: There is a relative overeexpression of both collagen type I and collagen type III mRNAs but not fibronectin in leiomyomas compared to myometrium from the same uteri of women treated with GnRH-a.

Conclusion: Leiomyomas obtained from women treated with GnRH-a show an up-regulation of collagens type I and type III similar to that seen in leiomyomas obtained from women in the proliferative phase of their menstrual cycles.

Key Words: Leiomyomas • myometrium • collagen • gonadotropin-releasing hormone agonist

Journal of the Society for Gynecologic Investigation, Vol. 5, No. 1, 44-47 (1998)
DOI: 10.1177/107155769800500109
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