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Mesothelium Expression of Integrins In Vivo and In Vitro

Department of Obstetrics and Gynecology, University of Texas Health Science Center at San Antonio, San Antonio, Texas; Department of Pathology, Louisiana State University, School of Medicine, New Orleans, Louisiana; Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive. San Antonio, TX 78284-7836

Iris A. Montoya-Rodriguez, BS

Dena M. Miller, MA

Barbara G. Schneider, PhD

Robert S. Schenken, MD

Department of Obstetrics and Gynecology, University of Texas Health Science Center at San Antonio, San Antonio, Texas; Department of Pathology, Louisiana State University, School of Medicine, New Orleans, Louisiana

Objective: To characterize the expression of subunits of integrin adhesion molecules in peritoneal tissue in vivo and in vitro.

Method: Peritoneum from the anterior abdominal wall (n = 22) and the serosa of the posterior uterus (n = 11) was obtained from women of reproductive age without endometriosis who were undergoing surgery for benign conditions. Immunohistochemical studies were performed on serial of peritoneum from the anterior abdominal wall, the uterine serosa, mesothelial monolayer cultures, and peritoneum explants from the abdominal wall using monoclonal antibodies to subunits of integrin adhesion molecules. Electron microscopy was performed to localize these adhesion molecules in the mesothelium.

Results: The mesothelial expression of integrin subunits was identical in the anterior peritoneum and uterine serosa. In vivo the mesothelium strongly expressed ₂ and ₃ and variably expressed ₆. In the monolayer cultures there was moderate/strong staining for _{2, 3,} and _5; and _{v; 6} was variably expressed. The ultrastructure of the mesothelium was unique in the anterior peritoneum, uterine serosa, and the nonolayer cultures. The integrin subunits were distributed throughout the cytoplasm, were expressed in the plasma membrane, and were present on the surface (ie, towards the peritoneal cavity) of the mesothelium.

Conclusion: Integrins are expressed by the mesothelium of the peritoneum. The mesothelium expression of integrins in vivo differs from that of the mesothelium integrin expression in monolayer culture and explant culture.

Key Words: Integrins • adhesion • peritoneum • mesothelium • monolayer • culture

Journal of the Society for Gynecologic Investigation, Vol. 5, No. 2, 87-93 (1998)
DOI: 10.1177/107155769800500207


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