Journal of the Society for Gynecologic Investigation

 

Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

http://mc.manuscriptcentral.com/rsci

SAGETRACK

Sign In to gain access to subscriptions and/or personal tools.
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Osborne, J.
Right arrow Articles by Baker, V. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Osborne, J.
Right arrow Articles by Hu, C.
Right arrow Articles by Hawley, C.
Right arrow Articles by Underwood, L. J.
Right arrow Articles by O'Brien, T. J.
Right arrow Articles by Baker, V. V.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of the Society for Gynecologic Investigation, Vol. 5, No. 5, 277-280 (1998)
DOI: 10.1177/107155769800500509

Expression of HOXDIO Gene in Normal Endometrium and Endometrial Adenocarcinoma

Janet Osborne, MD

Division of Gynecologic Oncology, University of MichigaN. Ann Arbor, Michigan and Departments of Obsteries and Gynecology, and Biocgemistry and Moclecular Biology, University of Arkansas for Medical Sciences, Little Rock, Arkansas; Lehigh Valley Women's Cancer Center, 1011 Pond Road, Allentown, PA 18104

Changzi Hu, MD

Colleen Hawley, BS

Lowell J. Underwood, BS

Timothy J. O'Brien, PhD

Vicki V. Baker, MD

Division of Gynecologic Oncology, University of MichigaN. Ann Arbor, Michigan and Departments of Obsteries and Gynecology, and Biocgemistry and Moclecular Biology, University of Arkansas for Medical Sciences, Little Rock, Arkansas

Objective: Hox genes encode DNA transcription regulatory that contain a conserved 61 amino acid protein called the homeodomain. Although best known for their role in cellular differentiation during embryonic development, aberrant expression of these genes has been associated with hematologic and solid neoplasms. The purpose of this study was to determine the relative expression of HOXD10 in huamn endometrial adenocarcionmas.

Methods: mRNA was isolated from 7 normal endometrial specimens and 28 endometrial adenocarcinoma specimens. cDNA was synthesized using randon hexamer primers. The expression of HOXD10 relative to ßtubulin (internal control) was assessed by densitometric comparison of co-amplified Phosphorus-32 (32P) labeled gene products separated by agarose gel electrophoesis. Direct sequencing of purified HOXD10 polymerase chain reaction product was also performed.

Results: The sequence of the purified HOXD10 product corresponds to the known DNA sequence reported in the National Institutes of Health Gene Bank. mRNA expression of HOXD10 relative to ß-tubulin is significantly lower in endometrial carcinomas than in normal endometrium. Furthermore, the ratio of HOXD10 to ß-tubulin expression varies inversely with the histologic grade of the tumor (P = .0009).

Conclusion: Cancer is a multistep proces involving aberrant expression of genes that regulate cell growth and differentiation. Human HOXD10 gene expression is altered in endometrial carcinoma and varies with the histologic grade of differentiation. This observation supports the theory that homeobox genes play a role in oncogenesis.

Key Words: Homeobox genes • HOXD10 • oncogenesis • endometrial cancer


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?