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Journal of the Society for Gynecologic Investigation
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Genome Scanning Detects Amplification of the Cathepsin B Gene (CtsB) in Transformed Rat Ovarian Surface Epithelial Cells

Abbas Abdollahi, PhD

Lori A. Getts, PhD

Gonosuke Sonoda, PhD

Paul D. Miller, PhD

Takahiro Taguchi, PhD

Andrew K. Godwin, PhD

Joseph R. Testa, PhD

Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, and Department of Chemistry, Lehigh University, Bethlehem, Pennsylvania

Thomas C. Hamilton, PhD

Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, and Department of Chemistry, Lehigh University, Bethlehem, Pennsylvania; tc_hamilton{at}fccc.edu

Objective: To isolate a portion of the amplicon inferred to be present in a malignant rat tumor cell line, NuTu 26, by the presence of a homogeneously staining chromosomal region (hsr) and identify genes embedded within it.

Methods: Genome scanning was used to identify an EcoRI fragment (8.6 kbp) within the amplified region of the NuTu 26 genome using a recently identified rat repetitive sequence, OST17 as a probe. The 8.6 kbp amplified fragment was sequenced and used as starting material to obtain additional sequence information by screening a P1 clone-derived DNA library to identify any genes likely embedded in the amplicon. Use of the microdissected hsr as a probe for fluorescence in situ hybridization (FISH) and application of Southern, Northern, and Western blot analysis confirmed the amplification of this region in the NuTu 26 genome.

Results: The cathepsin B gene was within the amplicon of the hsr-containing marker chromosome of NuTu 26. FISH analysis and chromosomal banding further revealed that the marker chromosome was a derivative of chromosomes 4 and 15, ie, der(15)t(4;15).

Conclusion: Cathepsin B gene amplification may contribute to some aspect of the biology of ovarian cancer. This concept is strengthened by the finding that the gene is overexpressed frequently in independently transformed rat ovarian surface epithelial cells.

Key Words: Cancer • ovary • cathepsin B • tumor invasiveness

Journal of the Society for Gynecologic Investigation, Vol. 6, No. 1, 32-40 (1999)
DOI: 10.1177/107155769900600108
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