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Journal of the Society for Gynecologic Investigation
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Endothelial Monocyte-Activating Polypeptide-2 Is Increased in Pregnancy but Is Not Further Increased in Preeclampsia

Robert P. Wellings, PhD

Gendie E. Lash, PhD

J. Clifford Murray, PhD

Maarten Tas, PhD

Wynne Ward

Alison J. Trew, PhD

University of Nottingham Division of Obstetrics, Midwifery and Gynaecology, School of Human Development, and Division of Clinical Oncology, School of Clinical Laboratory Science, Nottingham City Hospital, Nottingham, United Kingdom

Philip N. Baker, DM

School of Human Development, Division of Obstetrics, Midwifery and Gynaecology, University of Nottingham, Nottingham City Hospital. Hucknall Road, Nottingham. NG5 IPB, United Kingdom philip.baker{at}nottingham.ac.uk

Objectives: Endothelial monocyte-activating polypeptide-2 (EMAP-2) is a novel protein that demonstrates potent proinflammatory activity in vivo and in vitro. The purpose of this study was to investigate the expression of EMAP-2 in normal pregnancy and in pregnancies complicated with preeclampsin and to test the hypothesis that EMAP-2 is a causative agent of the endothelial activation of preeclampsia.

Methods: Expression of EMAP-2 in the placenta was investigated using reverse transcriptase-polymerase chain reaction to detect mRNA, and immunohistochemistry with an EMAP-2-specific polyclonal antiserum was carried out to detect protein. Levels of circulating EMAP-2 protein were measured in the blood of nonpregnant, normal pregnant, and preeclamptic individuals using a specific enzyme-linked immunoabsorbent assay and the molecular forms present assessed by Western blotting.

Results: EMAP-2 is transcribed and translated by the placenta thoughout pregnancy and in preeclampsia and can be detected in the plasma of nonpregnant and pregnant individuals. Levels of circulating EMAP-2 antigen are raised in pregnancy compared with nonpregnant controls; however, levels in patients with preeclampsia are identical to those in normal pregnant individuals.

Conclusion: While circulating levels of EMAP-2 are increased in pregnancy, there is no evidence that EMAP-2 is involved directly in the pathogenesis of preeclampsia.

Key Words: Preeclampsia • endothelial monocyte-activating polypeptide-2 • pregnancy • cytokine

Journal of the Society for Gynecologic Investigation, Vol. 6, No. 3, 142-146 (1999)
DOI: 10.1177/107155769900600305
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