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Inhibitory Effect of Interleukin-8 on the Secretion of Platelet-Activating Factor Acetylhydrolase by Human Decidual Macrophages

Hisashi Narahara, MD

Department of Biochemistry and Obstetrics and Gynecology, the University of Texas Southwestern Medical Center, Dallas, Texas

John M. Johnston, PhD

Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas Texas; johnston{at}grnctr.swmed.edu

Objective: To determine whether interleukin-8 (IL-8) regulates platelet-activating factor acetylhydrolase (PAF-AH) secretion by decidual cells in vitro.

Methods: Decidual macrophages were obtained from human term decidual tissue by enzymic digestion and Ficoll-Paque centrifugation. The effect of IL-8 and phorbol esters on the secretion of PAF-acetylhydrolase by these cells was examined.

Results: IL-8 inhibited PAF-AH secretion by decidual macrophages in a dose-dependent manner. The inhibition was reversed partially by antibodies against tumor necrosis factor- and IL-1ß. IL-8-induced inhibition was blocked partially by the protein kinase C (PKC) inhibitors, sphingosine, and H-7 1-(5-isoquinoline sulfonyl)-2-methylpiperazine. A PKC activator, 12-O-tetradecanoyl phorbol 13-acetate, decreased PAF-AH secretion.

Conclusion: IL-8 may increase the PAF concentration in the decidua via its inhibitory effect on PAF-AH secretion by decidual macrophages. IL-8-induced inhibition of enzyme secretion may have been mediated in part by PKC-dependent signal transduction.

Key Words: Platelet-activating factor acetylhydrolase • decidual macrophage • interleukin-8

Journal of the Society for Gynecologic Investigation, Vol. 6, No. 6, 328-332 (1999)
DOI: 10.1177/107155769900600609


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