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Journal of the Society for Gynecologic Investigation
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Expression of 17ß-Hydroxysteroid Dehydrogenase Type 5 in Human Ovary: A Pilot Study

Ke-nan Qin, MD

Pritzker School of Medicine, University of Chicago Children's Hospital, University of Chicago, Chicago, Illinois

Robert L. Rosenfield, MD

Pritzker School of Medicine, University of Chicago Children's Hospital, University of Chicago, Chicago, Illinois; robros{at}peds.bsd.uchicago.edu

Objective: Conversion of androstenedione to testosterone, the most potent androgen secreted by the ovary, is carried out by androgenic 17ß-hydroxysteroid dehydrogenase (17ß-HSD) activity. The molecular basis for this is unclear. We tested the hypothesis that type 5 17ß-HSD (17ß-HSD5) is responsible for testosterone formation from androstenedione in the human ovary.

Methods: We used primers specific for each type of 17ß-HSD to identify quantitatively and directly sequence the polymerase chain reaction products of a human ovary library.

Results: 17ß-HSD1, 17ß-HSD4, and 17ß-HSD5 were detected in the library lysate, but not 17ß-HSD2 or 17ß-HSD3. 17ß-HSD5 was the predominant androgenic form of 17ß-HSD expressed in human ovary.

Conclusion: These data suggest that 17ß-HSD5 may play a major role in testosterone biosynthesis by the human ovary. Further investigation of the regulation of 17ß-HSD5 gene expression is warranted with regard to ovarian testosterone secretion in normal and abnormal states of ovarian function, such as polycystic ovary syndrome.

Key Words: 17ß-hydroxysteroid dehydrogenase • ovarian testosterone biosynthesis • polycystic ovary syndrome

Journal of the Society for Gynecologic Investigation, Vol. 7, No. 1, 61-64 (2000)
DOI: 10.1177/107155760000700109
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