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Reproductive Sciences
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Importance of the Renal Nerves for Basal and Stimulated Renin mRNA Levels in Fetal and Adult Ovine Kidneys

Hiroyuki Ito, MD

Jinjuan Wang, MD

Jack W. Strandhoy, PhD

Department of Physiology and Pharmacology, and Department of Obstetrics and Gynecology and Perinatal Research Laboratories, Wake Forest University School of Medicine, Winston-Salem, North Carolina

James C. Rose, PhD

Department of Physiology and Pharmacology, and Department of Obstetrics and Gynecology and Perinatal Research Laboratories, Wake Forest University School of Medicine, Winston-Salem, North Carolina; Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157jimrose{at}wfubmc.edu

OBJECTIVE: To investigate the effect of renal denervation on renin mRNA levels in fetal and nonpregnant adult ovine renocortical tissue and in isolated juxtaglomerular cells under basal conditions and after stimulation.

METHODS: The left kidney was denervated and the right kidney subjected to a sham procedure in nine ovine fetuses (136-141 days' gestation) and 20 nonpregnant ewes. After 5-7 days the denervated and intact kidneys were obtained, and renin-containing renal cortical cells were isolated and cultured overnight. Then cells were treated with isoproterenol, forskolin, or isomethylbutyl xanthine (IBMX) for 4 hours. Total RNA was isolated and renin mRNA measured by RNase protection assay. Cyclic adenosine monophosphate (cAMP) levels were measured in the incubation medium with a competitive enzyme immunoassay.

RESULTS: In adults, basal renin mRNA levels were significantly lower in denervated than in sham-operated kidneys. No diference was noted between denervated and intactfetal kidneys. Renin mRNA levels were significantly higher in fetal than in adult kidney tissue, and cells from fetuses had greater increases in renin mRNA after stimulation than did cells from adults. Fetal cells also released more cAMP into the incubation medium, and there was a correlation between cAMP and renin mRNA levels.

CONCLUSIONS: The data indicate the effects of renal denervation on renin mRNA expression in the kidney are age dependent and that thefetus in late gestation has a mechanismfor maintaining renin mRNA levels after denervation, which is absent or nonfunctional in the adult.

Key Words: Renin • renal nerves • fetal kidney • renin mRNA • cAMP

Reproductive Sciences, Vol. 8, No. 6, 327-333 (2001)
DOI: 10.1177/107155760100800604
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