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Experimental Endometriosis in the Rat Is Correlated With Colonic Motor Function Alterations but Not With Bacterial Load

Department of Physiology and Pharmacology, Ponce School of Medicine, Ponce, Puerto Rico, cappleyard{at}psm.edu

Myrella L. Cruz, BS, MT

Department of Physiology and Pharmacology, Ponce School of Medicine, Ponce, Puerto Rico

Edelmarie Rivera, BS

Department of Physiology and Pharmacology, Ponce School of Medicine, Ponce, Puerto Rico

Gerardo A. Hernandez, MT, PhD

Department of Physiology and Pharmacology, Ponce School of Medicine, Ponce, Puerto Rico

Idhaliz Flores, PhD

Department of Microbiology Ponce School of Medicine, Ponce, Puerto Rico

Endometriosis commonly presents with symptoms that mimic chronic gastrointestinal disorders. The authors used the autotransplantion model of endometriosis in rats to investigate the possible underlying mechanisms. After the rats were killed, the presence of endometriotic vesicles, colonic inflammation, and white blood cell (WBC) numbers in the peritoneal fluid was determined. Sections of colon and of jejunum were collected for measurement of myeloperoxidase (MPO) activity and bacterial counts, and isometric recording in response to acetylcholine was measured in segments of longitudinal and circular smooth muscle. Experimental animals had significantly more colonic damage, MPO activity, and WBC numbers than controls did. There was no significant difference in the total bacterial load; however, experimental animals demonstrated an increased tension in the longitudinal muscle, which correlated with WBC numbers and colonic damage. In summary, this study presents evidence for a significant effect of peritoneal endometriosis on colonic function and integrity, which may help explain the gastrointestinal symptoms associated with this disease.

Key Words: Endometriosis • animal model • gastrointestinal symptoms • colonic inflammation • motility.

References

• Zwas FR, Lyon DT Endometriosis: an important condition in clinical gastroenterology. Dig Dis Sci. 1991;36:353-364.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Prystowsky JB, Stryker SJ, Ujiki GT, Poticha SM Gastrointestinal endometriosis. Incidence and indications for resection. Arch Surg. 1988;123:855-858.[Abstract/Free Full Text]
• Shah M., Tager D., Feller E. Intestinal endometriosis masquerading as a common digestive disorder. Arch Intern Med. 1995;155:977-980.[Abstract/Free Full Text]
• Skoog SM, Foxx-Orenstein AE, Levy MJ, Session DR Intestinal endometriosis: the great masquerader. Curr Gastroenterol Rep. 2004;6:405-409.[CrossRef][Medline] [Order article via Infotrieve]
• Boulton R., Chawla MH, Poole S., Hodgson HG, Barrison IG Ileal endometriosis masquerading as Crohn's ileitis. J Clin Gastroenterol. 1997;25:338-342.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Langlois NE, Park KG, Keenan RA Mucosal changes in the large bowel with endometriosis: a possible cause of misdiagnosis of colitis? Hum Pathol. 1994;25:1030-1034.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Lea R., Bancroft K., Whorwell PJ Irritable bowel syndrome, chronic pelvic inflammatory disease and endometriosis: a comparison of symptomatology. Eur J Gastroenterol Hepatol. 2004;16:1269-1272.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Blumberg RS, Strober W. Prospects for research in inflammatory bowel disease. JAMA. 2001;285:643-647.[Abstract/Free Full Text]
• Sinha AA, Lopez T., McDevitt HO Autoimmune diseases: the failure of self disease tolerance. Science. 1990;248:1380-1388.[Abstract/Free Full Text]
• Sartor RB Current concepts of the etiology and pathogenesis of ulcerative colitis and crohn's disease. Inflamm Bowel Dis. 1995;24:475-501.
• Drossman DA, Camilleri M., Mayer EA,WhiteheadWE.AGA technical review on irritable bowel syndrome. Gastroenterology. 2002;123:2108-2131.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Mayer EA, Naliboff BD, Chang L. Basic pathophysiologic mechanism in irritable bowel syndrome. Dig Dis. 2001;19: 212-218.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Yantiss RK, Clement PB, Young RH Endometriosis of the intestinal tract: a study of 44 cases of a disease that may cause diverse challenges in clinical and pathologic evaluation. Am J Surg Pathol. 2001;25:445-554.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Mathias JR, Franklin R., Quast DC, et al. Relation of endometriosis and neuromuscular disease of the gastrointestinal tract: new insights. Fertil Steril. 1998;70:81-88.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Anaf V., Simon P., El Nakadi I., et al. Relationship between endometriotic foci and nerves in rectovaginal endometriotic nodules. Hum Reprod. 2000;15:1744-1750.[Abstract/Free Full Text]
• Coronado C., Franklin RR, Lotze EC, Bailey HR, Valdes CT Surgical treatment of symptomatic colorectal endometriosis. Fertil Steril. 1990;53:411-416.[Web of Science][Medline] [Order article via Infotrieve]
• Vernon MW, Beard JS, Graves K.,Wilson EA Classification of endometriotic implants by morphologic appearance and capacity to synthesize prostaglandin F. Fertil Steril. 1986;46: 801-806.[Web of Science][Medline] [Order article via Infotrieve]
• Anaf V., Chapron C., El Nakadi I.,De Moor V., Simonart T., Noel JC Pain, mast cells, and nerves in peritoneal, ovarian, and deep infiltrating endometriosis. Fertil Steril. 2006;86:1336-1343.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Rojas-Cartagena C., Appleyard CB, Santiago OI, Flores I. Experimental intestinal endometriosis is characterized by increased levels of soluble TNFRSF1B and downregulation of Tnfrsf1a and Tnfrsf1b gene expression. Biol Reprod. 2005;73: 1211-1218.[Abstract/Free Full Text]
• Hernández GA, Appleyard CB Bacterial load in animal models of acute and chronic "reactivated" colitis. Digestion. 2003; 67:161-169.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Bailey MT, Coe CL Endometriosis is associated with an altered profile of intestinal microflora in female rhesus monkeys. Hum Reprod. 2002;17:1704-1708.[Abstract/Free Full Text]
• Schultsz C.,Van Den Berg F., Ten Kate F., Tytgat G., Dankert J. The intestinal mucus layer from patients with inflammatory bowel disease harbors high numbers of bacteria compared with controls. Gastroenterology. 1999;117:1089-1097.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Hosseini JM, Goldhill JM, Bossone C., Pineiro-Carrero V., Shea-Donohue T. Progressive alterations in circular smooth muscle contractility in TNBS-induced colitis in rats. Neurogastroenterol Motil. 1999;11:347-356.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Porras M., Martin MT, Soler M., Vergara P. Intestinal motor disorders associated with cyclical bacterial overgrowth in a rat model of enteritis. Am J Physiol. 2004;287:58-64.
• Vernon MW,Wilson EA Studies on the surgical induction of endometriosis in the rat. Fertil Steril. 1985;44:684-694.[Web of Science][Medline] [Order article via Infotrieve]
• Ingelmo MR, Quereda F., Acién P. Intraperitoneal and subcutaneus treatment of experimental endometriosis with recombinant human interferon--2b in a murine model. Fertil Steril. 1999;71:907-911.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Appleyard CB, Wallace JL Reactivation of hapten-induced colitis and its prevention by anti-inflammatory drugs. Am J Physiol. 1995;269:G119-G125.[Web of Science][Medline] [Order article via Infotrieve]
• Bradley P., Priebat D., Christensen R., Rothstein G. Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker.J Invest Dermatol. 1982; 78:206-209.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Appleyard CB, Morales M., Percy WH Regional variations in neurokinin receptor subtype contributions to muscularis mucosae and epithelial function in rat colon. Dig Dis Sci. 2006;51:506-516.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Myers BS, Martin JS, Dempsey DT, Parkman HP, Thomas RM, Ryan JP Acute experimental colitis decreases colonic circular smooth muscle contractility in rats. Am J Physiol. 1997;273:G928-G936.[Web of Science][Medline] [Order article via Infotrieve]
• Bossone C., Hosseini JM, Pinero-Carrero V., Shea-Donohue T. Alterations in spontaneous contractions in vitro after repeated inflammation of rat distal colon. Am J Physiol. 2001;280: G949-G957.[Web of Science]
• Matuk R., Crawford J., Abreu MT, Targan SR, Vasiliauskas EA, Papadakis KA The spectrum of gastrointestinal toxicity and effect on disease activity of selective cyclooxygenase-2 inhibitors in patients with inflammatory bowel disease. Inflamm Bowel Dis. 2004;10:352-356.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Sharma SC, Barry-Kinsella C., Cottell E., Harrison R.F. A midluteal phase comparison of peritoneal fluid volume and its content of PGF2 alpha and PGE2 in women with minimal stage endometriosis and a normal pelvis. Prostaglandins 1994;47:9-16.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Wu MH, Shoji Y., Wu MC, et al. Suppression of matrix metalloproteinase-9 by prostaglandin E(2) in peritoneal macrophage is associated with severity of endometriosis. Am J Pathol. 2005;167:1061-1069.[Abstract/Free Full Text]
• Grasa L.,Arruebo MP, Plaza MA, Murillo MD PGE(2) receptors and their intracellular mechanisms in rabbit small intestine. Prostaglandins Other Lipid Mediat. 2006;79:206-217.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Van Hoogmoed LM, Snyder JR, Harmon F. In vitro investigation of the effect of prostaglandins and nonsteroidal anti-inflammatory drugs on contractile activity of the equine smooth muscle of the dorsal colon, ventral colon, and pelvic flexure. Am J Vet Res. 2000;61:1259-1266.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Burakoff R., Percy WH Studies in vivo and in vitro on effects of PGE2 on colonic motility in rabbits. Am J Physiol. 1992;262(1 pt 1):G23-G29.[Web of Science][Medline] [Order article via Infotrieve]
• Bauer AJ, Boeckxstaens GE Mechanisms of postoperative ileus. Neurogastroenterol Motil. 2004;16(suppl 2):54-60.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Fukuda H., Tsuchida D., Koda K., Miyazaki M., Pappas TN, Takahashi T. Impaired gastric motor activity after abdominal surgery in rats. Neurogastroenterol Motil. 2005;17:245-250.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Winnard KP, Dmitrieva N., Berkley KJ Cross-organ interactions between reproductive, gastrointestinal, and urinary tracts: modulation by estrous stage and involvement of the hypogastric nerve. Am J Physiol Regul Integr Comp Physiol. 2006;291:R1592-R1601.[CrossRef]
• Ustinova EE, Fraser MO, Pezzone MA Colonic irritation in the rat sensitizes urinary bladder afferents to mechanical and chemical stimuli: an afferent origin of pelvic organ cross-sensitization. Am J Physiol Renal Physiol. 2006;290:1478-1487.[CrossRef]
• Houdeau E., Larauche M., Monnnerie R., Bueno L., Fioramonti J. Uterine motor alterations and estrous cycle disturbances associated with colonic inflammation in the rat. Am J Physiol. 2005;288:630-637.
• Berkley KJ, Rapkin AJ, Papka RE The pains of endometriosis. Science. 2005;308:1587-1589.[Abstract/Free Full Text]
• Berkley KJ, Dmitrieva N., Curtis KS, Papka RE Innervation of ectopic endometrium in a rat model of endometriosis. Proc Natl Acad Sci U S A. 2004;101:11094-11098.[Abstract/Free Full Text]
• Grummer R. Animal models in endometriosis research. Hum Reprod Update. 2006;12:641-649.[Abstract/Free Full Text]
• Story L., Kennedy S. Animal studies in endometriosis: a review. ILAR J. 2004;45:132-138.[Web of Science][Medline] [Order article via Infotrieve]
• Sharpe-Timms KL Using rats as a research model for the study of endometriosis. Ann N Y Acad Sci. 2002;955:318-327.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Cason AM, Samuelsen CL, Berkley KJ Estrous changes in vaginal nociception in a rat model of endometriosis. Horm Behav. 2003;44:123-131.[CrossRef][Medline] [Order article via Infotrieve]
• Uchiide I., Ihara T., Sugamata M. Pathological evaluation of the rat endometriosis model. Fertil Steril. 2002;78:782-786.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Flores I., Rivera E., Ruiz LA, Santiago OI, Vernon MW, Appleyard CB Molecular profiling of experimental endometriosis identified gene expression patterns in common with human disease. Fertil Steril. 2007;87:1180-1199.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]

Reproductive Sciences, Vol. 14, No. 8, 815-824 (2007)
DOI: 10.1177/1933719107309722


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This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Appleyard, C. B. Articles by Flores, I. Search for Related Content PubMed PubMed Citation Articles by Appleyard, C. B. Articles by Cruz, M. L. Articles by Rivera, E. Articles by Hernandez, G. A. Articles by Flores, I. Social Bookmarking                         What's this?