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Reproductive Sciences
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Article

Circulating Platelet-derived and Placenta-derived Microparticles Expose Flt-1 in Preeclampsia

Christine Anne Lok, M.D.*, Anita Böing1, Ian Sargent2, Suren Sooranna3, Joris Van der Post1, Rienk Nieuwland1, and Augueste Sturk1

1 Academic Medical Center, Amsterdam, The Netherlands
2 University of Oxford, The Women's Centre, John Radcliffe Hospital, Oxford, United Kingdom
3 Imperial College School of Medicine, Chelsea and Westminster Hospital, London, United Kingdom

* To whom correspondence should be addressed. E-mail: c.a.lok{at}amc.uva.nl.


  Abstract

Background. Flt-1 is secreted by various cells and elevated concentrations are present in preeclampsia affecting vascular function. Microparticles from these cells may expose Flt-1. We evaluated whether Flt-1 is microparticle-associated in preeclampsia, and established the origin of Flt-1-exposing microparticles. Methods. The concentration of Flt-1 was measured in samples from preeclamptic patients, pregnant and nonpregnant women by enzyme-linked immunosorbent assay. Microparticles were analyzed by flow cytometry. Western blot determined the different forms of Flt-1. Results. Noncell bound Flt-1 was elevated in preeclampsia compared to controls. A fraction (5%) was associated with microparticles in preeclampsia. Flt-1-exposing microparticles were increased in preeclampsia compared to normotensive pregnancy (p = 0.02). Full-length Flt-1, was identified in microparticles of platelet and placental origin. Conclusion. Full-length Flt-1 is associated with platelet and placenta-derived microparticles. Possibly, the presentation of Flt-1 on the membrane of a microparticle might alter its function, particularly if it acts in synergism with other exposed vasoactive molecules.

First published on October 20, 2008, doi:10.1177/1933719108324133

Reproductive Sciences 2008;15:1002.

A more recent version of this article appeared on December 1, 2008

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