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Reproductive Sciences
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Article

Recombinant Epidermal Growth Factor Maintains Follicular Ultrastructure and Promotes the Transition to Primary Follicles in Caprine Ovarian Tissue Cultured In Vitro

J. J. H. Celestino, M.S.*, J. B. Bruno, M.S., I. B. Lima-Verde, M.S., M. H. T. Matos, PhD, M. V. A. Saraiva, M.S., R. N. Chaves, M.S., F. S. Martins, M.S., L. F. Lima, Undergraduate Student, K. P. O. Name, M.S., C. C. Campello, PhD, J. R. V. Silva, PhD, S. N. Báo, PhD, and J. R. Figueiredo, PhD

* To whom correspondence should be addressed. E-mail: juliana_jales{at}yahoo.com.br.


  Abstract

We investigated the effects of epidermal growth factor on the survival and growth of caprine preantral follicles. Ovarian fragments were cultured for 1 and 7 days in enriched minimal essential medium with epidermal growth factor (0, 1, 10, 50, 100, or 200 ng/mL). Non-cultured and cultured tissues were processed for histological and ultrastructural studies. Results showed that after 7 days, the epidermal growth factor (1 and 10 ng/mL) maintained the percentage of normal follicles similar to control. An increase in the percentage of primary follicles was observed with 1, 10, and 50 ng/mL of epidermal growth factor compared to enriched minimal essential medium. Ultrastructural studies confirmed follicular integrity after 7 days in epidermal growth factor (1 and 10 ng/mL). In conclusion, the low concentrations of epidermal growth factor maintain caprine follicular viability and promote the transition from primordial to primary follicles.

First published on December 15, 2008, doi:10.1177/1933719108325756

Reproductive Sciences 2009;16:239.

A more recent version of this article appeared on March 1, 2009

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