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Reproductive Sciences
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Article

Mechanisms of Leukocyte Accumulation and Activation in Chorioamnionitis: Interleukin 1Beta and Tumor Necrosis Factor Alpha Enhance Colony Stimulating Factor 2 Expression in Term Decidua

Felice Arcuri, PhD, Paolo Toti, MD, Lynn Buchwalder, MS, Alessandra Casciaro, MS, Marcella Cintorino, MD, Frederick Schatz, PhD*, Basya Rybalov, PhD, and Charles J. Lockwood, MD

* To whom correspondence should be addressed. E-mail: frederick.schatz{at}yale.edu.


  Abstract

Chorioamnionitis is a major cause of prematurity as well as perinatal morbidity and mortality. The present study observed a marked increase in immunohistochemical staining for Colony Stimulating Factor 2 (CSF2; also known as granulocyte macrophage-colony stimulating factor), a potent neutrophil and macrophage chemoattractant and activator, in the decidua of patients with CAM compared with controls (n = 8; P = .001). To examine the regulation of this CSF2, cultured decidual cells primed with estradiol (E2) or E2 plus medroxyprogesterone acetate, were exposed to tumor necrosis factor-{alpha} or interleukin-1{beta} and secreted CSF2 measured by ELISA. Levels of CSF2 in E2 plus MPA-treated cultures increased 18- and 245-fold following treatment with TNF or IL1B (n = 7, P < .05). Quantitative RT-PCR demonstrated parallel changes in mRNA levels. This study reveals that CSF2 is strongly expressed in decidua from patients with CAM and indicates TNF or IL1B as important regulators of CAM-related decidual leukocyte infiltration and activation.

First published on January 22, 2009, doi:10.1177/1933719108328609

Reproductive Sciences 2009;16:453.

A more recent version of this article appeared on May 1, 2009

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